Salmonella Typhimurium TTSS-2 deficient mig-14 mutant shows attenuation in immunocompromised mice and offers protection against wild-type Salmonella Typhimurium infection
1 School of Biotechnology, KIIT University, Bhubaneswar 751024, Odisha, India
2 National Centre for Cell Science, Ganeshkhind, Pune, India
BMC Microbiology 2013, 13:236 doi:10.1186/1471-2180-13-236Published: 22 October 2013
Additional file 1: Figure S1:
Evaluation of attenuation profile of mig14::aphT mutant in comparison to wild-type strain of Salmonella Typhimurium. Competitive index profile of mig-14::aphT mutant when compared against wild-type strain. n.s. = not significant; * = p < 0.05). Figure S2. Infection profile of mig14::aphT mutant in comparison to wild-type strain of Salmonella Typhimurium .Infection profile and systemic attenuation of mig14::aphT mutant. Bar indicates 200 μm. n.s. = not significant; * = p < 0.05). Figure S3. Flowcytometric analysis of T-cell population after Salmonella infection. The whole cells were isolated from the mLN of the vaccinated mice. The cells were then suspended in appropriate medium and processed for flow cytometric analysis (see materials and methods). The cells were detected by using specific conjugated antibodies against specific T-cells. Figure S4. Luminal and serum specific antibody responses in mice immunized with MT5 and MT4. Serum and gut wash from mice treated with PBS and vaccinated with MT4 and MT5 were collected, diluted to a highest dilution of 1:120 (serum) and 1:9 (gut wash). The presence of Salmonella specific IgG and secretory IgA were detected by bacterial flow cytometric (A) and Western blot (B). Each coloured line indicates data obtained from individual mice of respective group. The representative Western blot analysis of the antibody responses was done by developing the blots from the overnight cultures of MT5, MT4, SB300 (wt S. Typhimurium) and M1525 (S. Enteritidis; negative control) by using the sera and gut luminal sIgA of the immunized mice.
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