Figure 6.

AlgU with missense mutations induces decreased amount of alginate compared to wild type AlgU. PAO1, CF149 and CF28 algUs were cloned into pHERD20T vector, and conjugated into PAO1ΔalgU and PAO1miniCTX-PalgD-lacZ, respectively. Alginate production (μg/ml/OD600) and PalgD activity were measured after culture overnight on PIA plates supplemented with 300 μg/ml of carbenicillin. The values reported here represent an average of three independent experiments with standard error.

Yin et al. BMC Microbiology 2013 13:232   doi:10.1186/1471-2180-13-232
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