Figure 4.

Induction of PmucEactivity by cell wall stress. A. A 1/200 dilution of the PAO1::attB::PmucE-lacZ recombinant strain grown overnight was inoculated into LB media containing X-gal and the agents listed as follows, 1) LB (control), 2) triclosan 25 μg/ml, 3) tween-20 0.20% (v/v), 4) hydrogen peroxide 0.15%, 5) bleach 0.03%, 6) SDS 0.10%, 7) ceftazidimine 2.5 μg/ml, 8) tobramycin 2.5 μg/ml, 9) gentamicin 2.5 μg/ml, 10) colisitin 2.5 μg/ml, and 11) amikacin 2.5 μg/ml. B. Triclosan, SDS, and ceftazidimine were tested for the induction of the PmucE and PalgU promoters. The activities of the promoter fusions were measured by β-galactosidase activity as described in Methods.

Yin et al. BMC Microbiology 2013 13:232   doi:10.1186/1471-2180-13-232
Download authors' original image