Open Access Research article

Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae

Lauren C Liddell12, Glenn M Manthey1, Shannon N Owens3, Becky XH Fu4 and Adam M Bailis12*

Author Affiliations

1 Department of Molecular and Cellular Biology, Beckman Research Institute of the City of Hope, 91010 Duarte, CA, USA

2 Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, 91010 Duarte, CA, USA

3 Occidental College, 90041 Los Angeles, CA, USA

4 Department of Genetics, Stanford University School of Medicine, 94305 Stanford, CA, USA

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BMC Microbiology 2013, 13:229  doi:10.1186/1471-2180-13-229

Published: 14 October 2013

Additional files

Additional file 1: Table S1:

Saccharomyces cerevisiae strains used in this study. Table S2. Summary of quantitative data. Figure S1. A. Multiple amino acid sequence alignment of ScRad59 with ScRad52 and HsRad52. B. Molecular modeling of the proteins encoded by the rad59 missense alleles demonstrates that Rad59-Y92A is in a different structural motif. Figure S2. The unequal sister chromatid recombination (USCR) assay for measuring spontaneous homologous recombination between sister chromatids in haploid yeast. Figure S3. The loss of heterozygosity assay for measuring spontaneous Rad51-independent homologous recombination. Figure S4. LOH is the recombination product of a single-ended DSB, whereas HAR results from repair of a double-ended DSB. A) LOH results from the repair of a single-ended DSB by HR. B) HAR results from the repair of a double-ended DSB by HR.

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