Carbohydrate content in the cell wall of Paracoccidioides yeast cells exposed to OenB. The cell walls of Paracoccidioides that were either untreated (control) or treated with OenB were isolated. The alkali-soluble (AS) and alkali-insoluble (AI) fractions were separated. (A) Total amount of carbohydrates was measured in the AS and AI fractions. (B) The amount of 1,3-β-D-glucan polymer was estimated in the AI fraction. (C) The amount of N-acetylglucosamine (GlcNAc) residue was measured in the total cell wall fraction. All data were normalized relative to the control. Three independent experiments were performed. *Significantly increased amount (p ≤ 0.05).
Zambuzzi-Carvalho et al. BMC Microbiology 2013 13:227 doi:10.1186/1471-2180-13-227