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Open Access Highly Accessed Research article

Lipoproteins of slow-growing Mycobacteria carry three fatty acids and are N-acylated by Apolipoprotein N-Acyltransferase BCG_2070c

Juliane K Brülle1, Andreas Tschumi1 and Peter Sander12*

Author Affiliations

1 Institute of Medical Microbiology, University of Zurich, Gloriastrasse 30/32, CH-8006, Zurich, Switzerland

2 National Reference Laboratory for Mycobacteria (NRLM), Gloriastrasse 30, CH-8006, Zurich, Switzerland

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BMC Microbiology 2013, 13:223  doi:10.1186/1471-2180-13-223

Published: 5 October 2013

Additional files

Additional file 1: Figure S1:

Western blot analysis of purified lipoproteins of M. bovis BCG wildtype and Δlnt mutant strain.

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Additional file 2: Figure S2:

Sequence alignment of M. tuberculosis Rv2262c/Rv2261c and M. bovis BCG_2070c using EMBOSS Needle.

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Additional file 3: Figure S3:

Multiple sequence alignment of Lnt homologues using Clustal W2.

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Additional file 4: Table S1:

Conservation of essential residues in Lnt homologues.

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Additional file 5: Figure S4:

Disruption of Mycobacterium bovis BCG lnt (BCG_2070c).

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Additional file 6: Figure S5:

MALDI-TOF analysis of the N-terminal peptides of LprF.

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Additional file 7: Figure S6:

MALDI-TOF analysis of the N-terminal peptides of LppX.

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