Specificity of H7 antibody detection in the dual ELISA. Sera from different animals immunized with different subtypes of influenza viruses were collected 10 days after the 2nd immunization and normalized to a HI titer of 16 before tested in the dual ELISA. Inhibition above the cut-off value of 30% blocking was considered as positive; i.e. antibodies to H7 were present. The results were expressed as the arithmetic mean of percent blocking values. aH7N7: A/duck/Hokkaido/1/10; Ck: chicken; Gp: guinea pig; Ms: mouse; Bac: wildtype baculovirus immunized serum; Blank: preimmune serum. Dotted line: cut-off values.
He et al. BMC Microbiology 2013 13:219 doi:10.1186/1471-2180-13-219