Open Access Highly Accessed Research article

Detection and quantification of a mycorrhization helper bacterium and a mycorrhizal fungus in plant-soil microcosms at different levels of complexity

Florence Kurth1*, Katharina Zeitler1, Lasse Feldhahn1, Thomas R Neu2, Tilmann Weber3, Václav Krištůfek4, Tesfaye Wubet15, Sylvie Herrmann56, François Buscot157 and Mika T Tarkka157

Author Affiliations

1 Department Soil Ecology, UFZ - Helmholtz Centre for Environmental Research, Theodor-Lieser-Str. 4, 06120 Halle/Saale, Germany

2 Department River Ecology, UFZ - Helmholtz Centre for Environmental Research, Brückstraße 3a, 39114 Magdeburg, Germany

3 Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Auf der Morgenstelle 28, Tübingen, Germany

4 Biology Centre AS CR, v. v. i. - Institute of Soil Biology, Na Sádkách 7, 370 05 České Budějovice, Czech Republic

5 German Centre for Integrative Biodiversity Research, University of Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany

6 Department of Community Ecology, UFZ - Helmholtz Centre for Environmental Research, Theodor-Lieser-Str. 4, 06120 Halle/Saale, Germany

7 Institute of Biology, University of Leipzig, Johannisallee 21-23, 04103 Leipzig, Germany

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BMC Microbiology 2013, 13:205  doi:10.1186/1471-2180-13-205

Published: 11 September 2013

Additional files

Additional file 1:

Experimental setup for quantification of AcH 505 and P. croceum under different culture conditions.

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Additional file 2:

qRT-PCR melting and standard curves obtained using the AcH107 primer pair.

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Additional file 3:

qRT-PCR melting and standard curves obtained with the ITS-P primer pair.

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Additional file 4:

qRT-PCR melting and standard curves obtained with the ITSP1 primer pair.

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Additional file 5:

qRT-PCR melting and standard curves obtained with the Pilo127 primer pair.

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Additional file 6:

Correlation of AcH 505 and P. croceum biomass with qRT-PCR data.

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Additional file 7:

Statistical analysis relating to the quantification of the mycorrhization helper bacterium Streptomyces sp. AcH 505 and the mycorrhizal fungus Piloderma croceum in soil microcosms.

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Additional file 8:

Cryo-field emission scanning electron microscopy (cryo-FESEM) images.

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Additional file 9:

Confocal laser scanning microscopy (CLSM) images.

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Additional file 10:

eGFP labelling of Streptomyces sp. AcH 505.

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Additional file 11:

Visualisation of the Streptomyces sp. AcH 505 – Piloderma croceum interaction using confocal laser scanning microscopy.

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