|TNF-α secretion of LPS-stimulated J774 cells infected with F. tularensis|
|Strain||TNF-α secretion (pg/ml)a|
|-||708 ± 102|
|LVS||45.9 ± 8.9***|
|ΔpdpC||36.4 ± 7.5***|
|ΔiglA||1340 ± 126|
aF. tularensis-infected, or uninfected (-), J774 cells were incubated for 2 h with LPS. The average TNF-α secretion in pg/ml with standard errors of triplicate samples is shown, results are from one representative experiment out of three. A Student’s t-test revealed that there was no significant difference in TNF-α secretion between LVS and ΔpdpC mutant infected cells, but that cells infected with either strain had a significantly lower TNF-α secretion than uninfected cells (***: P < 0.001).
Lindgren et al.
Lindgren et al. BMC Microbiology 2013 13:20 doi:10.1186/1471-2180-13-20