Subcellular localization of PdpC. LVS whole-cell lysate was separated into soluble, inner membrane (IM) and outer membrane (OM) fractions using ultracentrifugation and Sarkosyl treatment. After separation by SDS-PAGE, the presence of PdpC in each fraction was determined by Western blot using polyclonal anti-PdpC antibodies. Antibodies recognizing IglC and PdpB were used as markers for soluble and inner membrane fractions, respectively.
Lindgren et al. BMC Microbiology 2013 13:20 doi:10.1186/1471-2180-13-20