Depletion of endogenous Tsg101 or Alix using specific siRNA does not inhibit WNV release. 293T cells were transfected with control, Alix or Tsg101 siRNA. 24 h post transfection cells were transfected again with respective siRNAs along with (A) WT HIV-1 pNL4-3 DNA (B) WT EIAV Gag DNA or (C) WNV-CPrME plus the Ren/Rep plasmids. Virus release was determined after radiolabeling and immunoprecipitation for HIV and WNV, via western blotting for EIAV and also by the rapid ren-luc based assay for WNV. Data represent mean ± SD from 3 independent experiments (A&C). For the ren-luc based WNV assay one representative of 3 independent experiments is shown.
Garg et al. BMC Microbiology 2013 13:197 doi:10.1186/1471-2180-13-197