Properties analysis of synthetic peptide PL10. (A) Specific reactivity of PL10 with antibody 4D10 (anti-DENV1-4 prM mAb). The synthetic peptide PL10 could react with mAb 4D10 but control antibody 4G2 (anti-flavivirus E mAb) could not. (B) The sensitivity binding assay of synthetic peptides PL10 and two control peptides (PH10 and PM10) with mAb 4D10. The synthetic peptide PL10 bound the antibody in a concentration-dependent manner, but two control peptides had no reactivity with 4D10. (C) ELISA reactivities of synthetic peptide PL10 with immunized mice sera. Synthetic peptide PL10 was recognized by anti-DENV1-4 mice sera, whereas it was not recognized by anti-JEV mice sera and normal mice sera (NMS). (D) Competitive inhibition of phage clone binding to mAb 4D10 by synthetic peptide PL10. Competitive ELISA was performed using PL10 as competitor of its corresponding phage clones. The percentage of inhibition is also shown. (E and F) ELISA reactivities of synthetic peptide PL10 with serum samples from 20 DENV2-infected patients (E) and 20 healthy adults (F). PH10 and PM10 were used as control. Data are expressed as means of at least three independent experiments. The error bars represent standard deviations (SD). If there is no error bar, it is not that no variations among three independent experiments but that the variations are too small to show in the figure. * P < 0.05 vs PL10 at 0.1 μg.
Luo et al. BMC Microbiology 2013 13:194 doi:10.1186/1471-2180-13-194