Characterization of prM mAb 4D10. (A, B and C) Cross-reactivity of 4D10 with four DENV serotypes and JEV (negative control antigen for the specificity of the antibody 4D10) determined by ELISA (A), western blot (B) and IFA (C). These results showed that only DENV1-4 infected C6/36 cells could be detected with 4D10 and 2H2 (positive control antibody) but not JEV infected cells. Normal mouse serum (NMS) had no such reactivity with all flaviviruses. (D) Competitive inhibition of DENV2 patient sera binding to DENV2 by mAb 4D10. Competitive ELISA was performed using 4D10 as competitor of DENV2 patient sera. The percentage of inhibition is also shown. Data are expressed as means of at least three independent experiments. The error bars represent standard deviations (SD). If there is no error bar, it is not that no variations among three independent experiments but that the variations are too small to show in the figure. * P < 0.05 vs 4D10.
Luo et al. BMC Microbiology 2013 13:194 doi:10.1186/1471-2180-13-194