Table 3

L. gasseri adhesion to saliva coated hydroxyapatite and aggregation in saliva
Parotid saliva Submandibular/sublingual saliva
L. gasseri Adhesion1 Aggregation2 Adhesion1 Aggregation2
Isolate B16 ++ ++ +++ +++
Isolate B1 + + ++ ++
Isolate L10 + ++ ++ +++
Isolate A241 + + ++ ++
Isolate A274 + ++ ++ +++
Type strain 31451T ++ ++ +++ +++

1 62.5×106 bacterial cells were added into each test well. + binding of <15% of added bacterial cells, ++ ≥15 to <20%, and +++ ≥20%.

2 – =aggregation score 0 (no visible aggregates), + aggregation score 1 (small uniform aggregates), ++ aggregation score 2 (more aggregates of slightly larger size than 1), +++ aggregation score 3 (more and slightly larger aggregates than 2) [30]. Adhesion buffer was used a negative control (score 0) and S. mutans strain Ingbritt as positive control (score +++) [18].

Romani Vestman et al.

Romani Vestman et al. BMC Microbiology 2013 13:193   doi:10.1186/1471-2180-13-193

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