Figure 4.

Western blot detection of saliva gp340 and MUC7 after L. gasseri treatment. (A) Upper panel shows detection of gp340 (using mAb143) and lower panel MUC7 (usig mAb LUM7-2) in parotid and submandibular/sublingual saliva alone or after incubation with L. gasseri isolate B16; (B) upper panel shows detection of gp340 and lower panel MUC7 in parotid and submandibular/sublingual saliva alone or after incubation with S. mutans strain Ingbritt. Numbers below lanes in panels A and B refer to the following contents: (1) Bacterial cells alone (−ve control), (2) Parotid saliva alone (+ve control), (3) Parotid saliva after bacteria incubation, (4) Bacteria incubated in parotid saliva, (5) Bacteria after SDS protein release, (6) Submandibular saliva alone (+ve control), (7) Submandibular saliva after bacteria incubation, (8) Bacteria incubated in submandibular saliva, (9) Bacteria after SDS protein release. (C) upper panel depicts detection of gp340 in parotid saliva alone and after incubation with five different L. gasseri isolates and the L. gasseri type strain; (D) upper panel depicts detection of gp340 and lower panel detection of MUC7 in submandibular/sublingual saliva alone and after incubation with five different L. gasseri isolates and the type strain. Numbers below lanes in panels C and D refer to the following contents: (1) Saliva alone (+ve control), (2) Saliva after L. gasseri CCUG31451T incubation, (3) Saliva after L. gasseri isolate A241 incubation, (4) Saliva after L. gasseri isolate A274 incubation, (5) Saliva after L. gasseri isolate B1 incubation, (6) Saliva after L. gasseri isolate B16 incubation, (7) Saliva after L. gasseri isolate L10 incubation.

Romani Vestman et al. BMC Microbiology 2013 13:193   doi:10.1186/1471-2180-13-193
Download authors' original image