RecU and PBP2 are encoded in the same operon. A – Schematic representation of the recU-pbp2 operon in the NCTC8325-4 wild-type strain (top) and the 8325-4recUi mutant strain (bottom) where the recU gene, including the RBS, was placed in the spa locus under the control of the IPTG inducible Pspac promoter (white flag). Subsequently, the first 165 codons of the native copy of recU were deleted. Black flags represent the promoters (P1 and P2) of the recU-pbp2 operon. B – Western blot analysis of PBP2 levels in control strain BCBHV008 and recU inducible mutant 8325-4recUi grown in the presence or absence of IPTG showing that PBP2 levels were not affected by recU deletion. FtsZ was used as an internal control of total protein loaded.
Pereira et al. BMC Microbiology 2013 13:18 doi:10.1186/1471-2180-13-18