Time-course analysis of variant and wildtype population distributions in biofilms. A time-course of the individual populations of CHA0, CHA19, SCV, and WS (A), and the SCV and WS in mixed co-culture with either CHA0 or CHA19 (B), was done over a period of 96 h to determine how quickly the variant populations were overtaking the biofilm. CHA0 and CHA19 are expressing YFP, SCV is expressing RFP and the WS is expressing CFP. The images shown are 3D reconstructions from the CLSM z-stacks and each image was acquired from a separate peg in the CBD. All images were acquired at the same resolution and scale bars in the bottom right of each panel represent 40 μm.
Workentine et al. BMC Microbiology 2013 13:175 doi:10.1186/1471-2180-13-175