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Molecular diversity and high virulence of Legionella pneumophila strains isolated from biofilms developed within a warm spring of a thermal spa

Zineddine Chaabna1, Françoise Forey2, Monique Reyrolle2, Sophie Jarraud23, Danièle Atlan4, Dominique Fontvieille1 and Christophe Gilbert4*

Author Affiliations

1 UMR CARRTEL, Université de Savoie-INRA, Le Bourget du Lac, F-73376, France

2 Centre de Biologie et Pathologie Est, Centre National de Référence des Légionelles, Bron, F-69677, France

3 Université Lyon, INSERM U851, Université Lyon 1, Lyon, F-69008, France

4 Université de Lyon, CNRS UMR 5240, Université Lyon1, Villeurbanne, F-69622, France

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BMC Microbiology 2013, 13:17  doi:10.1186/1471-2180-13-17

Published: 28 January 2013

Additional files

Additional file 1:

PFGE analysis of environmental and clinical Legionella pneumophila strains. Legionella DNA samples were digested with SfiI restriction enzyme for 16 h at 50°C. Fragments of DNA were separated in a 0.8% agarose gel prepared and run in 0.5x Tris-borate-EDTA buffer (pH 8.3) in a contour-clamped homogeneous field apparatus with a constant voltage of 150 V. Runs were carried out with increasing pulse times (2 to 25 s) at 10°C for 11 h and increasing pulse times (35 to 60 s) at 10°C for 9 h.

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Additional file 2:

Multiple alignment of mip sequences from environmental (mip1, mip2 and mip3) and clinical L. pneumophila sg1 strains. Clinical strains: Lp1Corby (NC009494.2), Lp1 Lens (NC006369.1), Lp1 Paris (NC006368) and Lp1 Philadelphia (AE017354.1).

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