Figure 2.

EMSA of MsvR homologues on their respective promoters. The gel wells are indicated (W). (a and b) EMSA to test binding of MaMsvR (a) and MthMsvR (b) to the MaMsvR promoter (Ma PmsvR, 10 nM), the MthMsvR/fpaA intergenic promoter region (Mth PmsvR/fpaA, 10 nM), and the Mth histone B promoter (Mth PhmtB, 10 nM). Each promoter has a control lane (-) that contains no protein, a binding reaction that contains either Ma or Mth MsvR (200 nM) in the absence of DTT (non-reduced, +), and a binding reaction that contains either Ma or Mth MsvR (200 nM) in the presence of 5 mM DTT (reduced, R). (c) EMSA assay (10 nM Ma PmsvR DNA) with decreasing concentrations of reduced MaMsvR (5 mM DTT) [monomer] 1 μM, 500 nM, 250 nM, 125 nM, 62.5 nM, 31.3 nM, 15.6 nM, 7.8 nM, and 3.9 nM. (d) EMSA assay (10 nM Mth PmsvR/fpaA DNA) with decreasing concentrations of reduced MaMsvR (5 mM DTT) [monomer] 1 μM, 500 nM, 250 nM, 125 nM, 62.5 nM, 31.3 nM, 15.6 nM, 7.8 nM, and 3.9 nM. (e) EMSA assay (10 nM Mth PmsvR/fpaA DNA) with decreasing concentrations of reduced MthMsvR (5 mM DTT) [monomer] 1 μM, 500 nM, 250 nM, 125 nM, 62.5 nM, 31.3 nM, 15.6 nM, 7.8 nM, and 3.9 nM.

Isom et al. BMC Microbiology 2013 13:163   doi:10.1186/1471-2180-13-163
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