Table 3

List of bacterial strains and plasmids used in this study
Strain or plasmid Relevant characteristics Reference or source
A. baumannii strains
R2 Wild-type clinical MDR isolate TTSH6013 624325/06 Network for Antimicrobial Resistance Surveillance (Singapore)
DB Wild-type clinical MDR isolate DB15354/07 Network for Antimicrobial Resistance Surveillance (Singapore)
R2ΔadeFGH R2 with deletion in adeFGH operon This study
R2ΔadeIJK R2 with deletion in adeIJK operon This study
R2ΔadeFGHΔadeIJK R2 with deletion in adeFGH and adeIJK operons This study
DBΔadeFGH DB with deletion in adeFGH operon This study
DBΔadeIJK DB with deletion in adeIJK operon This study
DBΔadeFGHΔadeIJK DB with deletion in adeFGH and adeIJK operons This study
E. coli strains
DH5α F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 phoA supE44 λ– thi-1 gyrA96 relA1 Invitrogen
S17-1 Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7, GmS [16]
Plasmids
pMo130 Suicide plasmid, xylE+, sacB+, KmR [8]
pwFRT-TelR Donor of tellurite resistance cassette [10]
pMo130-TelR pMo130 plasmid containing 3.26 kb XmaI-digested tellurite-resistance cassette from pwFRT-TelR This study
pMo130-TelR-P8(UP/DWN) pMo130-TelR containing a 1 kb UP fragment (promoterless adeL) and 1 kb DOWN fragment (3’ partial adeH) This study
pMo130-TelR-adeJ(Up/Down) pMo130-TelR containing a 1 kb UP fragment (5’ partial adeI) and 0.9 kb DOWN fragment (3’ partial adeK) This study

Amin et al.

Amin et al. BMC Microbiology 2013 13:158   doi:10.1186/1471-2180-13-158

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