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Monitoring the prevalence of viable and dead cariogenic bacteria in oral specimens and in vitro biofilms by qPCR combined with propidium monoazide

Ai Yasunaga1, Akihiro Yoshida1*, Kazumasa Morikawa2, Kenshi Maki2, Suguru Nakamura1, Inho Soh1, Shuji Awano1 and Toshihiro Ansai1

Author Affiliations

1 Division of Community Oral Health Development, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku Kitakyushu 803-8580, Japan

2 Division of Developmental Stomatognatic Function Science, Kyushu Dental University, Kitakyushu 803-8580, Japan

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BMC Microbiology 2013, 13:157  doi:10.1186/1471-2180-13-157

Published: 13 July 2013

Additional files

Additional file 1: Figure S1:

Standard curves for the qPCR assay were generated by the bacterial cell number and Ct value. (A) S. mutans. (B) S. sobrinus. The mean values of independent triplicate data are shown.

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