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Evidence for isolated evolution of deep-sea ciliate communities through geological separation and environmental selection

Alexandra Stock1, Virginia Edgcomb2, William Orsi2, Sabine Filker1, Hans-Werner Breiner1, Michail M Yakimov3 and Thorsten Stoeck1*

Author Affiliations

1 University of Kaiserslautern, School of Biology, Erwin-Schroedinger-Str. 14, D-67663 Kaiserslautern, Germany

2 Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA

3 Institute for Coastal Marine Environment, IAMC-CNR, Spianata S. Raineri, 86, 98122 Messina, Italy

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BMC Microbiology 2013, 13:150  doi:10.1186/1471-2180-13-150

Published: 8 July 2013

Additional files

Additional file 1: Figure S1:

Rarefaction curves of V4 SSU rRNA-amplicons that were assigned to ciliate genera for all eight samples.

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Additional file 2: Figure S2:

Proportion of rare versus abundant ciliate taxa. The number of detected taxa is opposed to the number of ciliate V4 SSU rRNA amplicons.

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Additional file 3: Table S1:

Number of ciliate V4 SSU rRNA-amplicons in each sample assigned to described ciliate genera. The assigned genus represents the best BLAST hit of assigned amplicons to NCBIs GenBank nucleotide database 187.

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Additional file 4: Table S2:

Keyplayer ciliate taxa with minimum and maximum sequence similarity to known species. Sequence similarities from Genbank BLASTn.

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