Open Access Highly Accessed Methodology article

Identification of species belonging to the Bifidobacterium genus by PCR-RFLP analysis of a hsp60 gene fragment

Loredana Baffoni1*, Verena Stenico1, Erwin Strahsburger2, Francesca Gaggìa1, Diana Di Gioia1, Monica Modesto1, Paola Mattarelli1 and Bruno Biavati1

Author Affiliations

1 Department of Agricultural Sciences, University of Bologna, viale Fanin 42, 40127, Bologna, Italy

2 Laboratorio de Microbiología Molecular y Biotecnología Ambiental, Departamento de Química and Center of Nanotechnology and Systems Biology, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile

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BMC Microbiology 2013, 13:149  doi:10.1186/1471-2180-13-149

Published: 1 July 2013

Additional files

Additional file 1: Figure S1:

Example of agarose gel electrophoresis of hsp60 amplicons from different bifidobacterial strains.

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Additional file 2: Figure S2:

Agarose gel electrophoresis of digested hsp60 DNA fragments with HaeIII (negative image). Lane1, ladder 20 bp (Sigma-Aldrich); Lane 2–6, B. animalis subsp.lactis strains Ra20, Ra18, F439, P23, P32; Lane 7–8, B. animalis subsp. animalis strains T169, T6/1; Lane 9, ladder 20 bp (Sigma-Aldrich).

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Additional file 3: Figure S3:

Agarose gel electrophoresis of digested hsp60 DNA fragments with HaeIII (negative image). Lane1, ladder 20 bp (Sigma-Aldrich); Lane 2–4, B. longum subsp. suis strains Su864, Su908, Su932; Lane 5–6, B. longum subsp. longum strains PCB133, ATCC 15707 (T); Lane 7–9, B. longum subsp. infantis strains ATCC 15697 (T), B7740, B7710; Lane 9, ladder 20 bp (Sigma-Aldrich).

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