Comparative analyses of the inflammatory infiltrate into the site of infection after SGE inoculation. BALB/c mice were inoculated i.d. once (SGE-1X-gray bars) or three times (SGE-3X–black bars) within the ear dermis with SGE (derived from 0.5 pair of salivary glands diluted in 10 μl of PBS/ear) or PBS (10 μl/ear-white bars). The mice were euthanized 24 h later, and ears were harvested for inflammatory infiltrate characterization. The total number of CD4+ T cells (A), CD8+ T cells (B), CD4+CD25+ cells (C); dendritic cells (D), macrophages (E) and neutrophils (F) present within the ears were identified by flow cytometry. Data represent the mean ± SEM and are representative of three independent experiments (n = 4). #P < 0.05 compared with PBS (control group). *P < 0.05 compared with the SGE-1X group.
Carregaro et al. BMC Microbiology 2013 13:102 doi:10.1186/1471-2180-13-102