Additional file 3.

Subcellular localization of δ-Ama40 fused with GFP. (Additional file 3: Figure S3) Permeabilized, stable transfected CL Brener epimastigotes were incubated with anti-PEPCK antibody and a secondary antibody conjugated to Alexa546. GFP (panels A and D), Alexa 546 (B and E) and merged (C and F) fluorescent images were obtained by confocal microscopy of parasites expressing δ-Ama40GFP as described in Figure 4. (Bar = 10 μm).

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Kangussu-Marcolino et al. BMC Microbiology 2013 13:10   doi:10.1186/1471-2180-13-10