Figure 3.

Amastin mRNA expression during the T. cruzi life cycle in different parasite strains. Total RNA was extracted from epimatigote (E), trypomastigote (T) and amastigote forms (A) from CL Brener, Y, G and Sylvio X-10. Electrophoresed RNAs (~10 μg/lane) were transferred to nylon membranes and probed with the 32P- labelled sequences corresponding to δ-amastin, δ-Ama40, β1- and β2-amastins (top panels). Bottom panels show hybridization of the same membranes with a fragment of the 24Sα rRNA.

Kangussu-Marcolino et al. BMC Microbiology 2013 13:10   doi:10.1186/1471-2180-13-10
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