Open Access Research article

Distinct genomic organization, mRNA expression and cellular localization of members of two amastin sub-families present in Trypanosoma cruzi

Monica Mendes Kangussu-Marcolino1, Rita Márcia Cardoso de Paiva2, Patrícia Rosa Araújo2, Rondon Pessoa de Mendonça-Neto2, Laiane Lemos1, Daniella Castanheira Bartholomeu3, Renato A Mortara4, Wanderson Duarte daRocha1* and Santuza Maria Ribeiro Teixeira2*

Author Affiliations

1 Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Rua Quinze de Novembro, 1299, 80060-000, Centro Curitiba, PR, Brazil

2 Departamento de Bioquímica e Imunologia, Av. Antônio Carlos, 6627, 31270-901, Pampulha Belo Horizonte, MG, Brazil

3 Departamento de Parasitologia Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901, Pampulha Belo Horizonte, MG, Brazil

4 Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, Brazil, 04021-001, São Paulo, Brazil

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BMC Microbiology 2013, 13:10  doi:10.1186/1471-2180-13-10

Published: 17 January 2013

Additional files

Additional file 1:

Comparative sequence analysis ofT. cruzi amastins. (Figure S1A) Percentages of amino acid identities among all T. cruzi amastin sequences present in the CL Brener and Sylvio X-10 genome databases. (Figure S1B) Conserved amino acid residues and conserved domains among sequences corresponding to all amastin genes present in the T. cruzi CL Brener genome are represented using the WebLogo software. The x axis depicts the amino acid position. The taller the letter the lesser the variability at the site. Predicted transmembrane domains are underlined.

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Additional file 2:

Amino acid sequences of delta- and beta-amastins. (Figure S2) Predicted amino acid sequences of one representative member of δ-amastin, δ-ama40, β1 and β2-amastins present in the T. cruzi CL Brener genome.

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Additional file 3:

Subcellular localization of δ-Ama40 fused with GFP. (Additional file 3: Figure S3) Permeabilized, stable transfected CL Brener epimastigotes were incubated with anti-PEPCK antibody and a secondary antibody conjugated to Alexa546. GFP (panels A and D), Alexa 546 (B and E) and merged (C and F) fluorescent images were obtained by confocal microscopy of parasites expressing δ-Ama40GFP as described in Figure 4. (Bar = 10 μm).

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Additional file 4: Table S1:

Amastin sequences presented in Figure 1.

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