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Open Access Highly Accessed Research article

Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria

Tao Ding1, Michael W Palmer2 and Ulrich Melcher1*

Author Affiliations

1 Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA

2 Department of Botany, Oklahoma State University, Stillwater, OK, 74078, USA

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BMC Microbiology 2013, 13:1  doi:10.1186/1471-2180-13-1

Published: 3 January 2013

Additional files

Additional file 1:

Table S1. Locations of sampling sites in the TGPP. Table S2. Dominant T-RFs from amplified 16S bacterial rDNA from three plant species. Table S3. Summary statistics for T-RFs calculated by each host species, sampling month and sampling date Table S5. Frequencies of all the T-RFs in 5 different host species and their average frequencies. Table S6. Average Proportion per Existence (APE) of all the T-RFs in 5 different host species.

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Additional file 2:

Figure S1. Comparison of two T-RFLP patterns of DdeI digestion products of the Asclepias viridis Sample 1 from Site 2 collected on June 16th, 2010, scanned on Aug 19th, 2010 (above) and Aug 30th 2010 (below). The T-RFLP patterns of the same sample scanned in different experiments were indistinguishable, indicating that the T-RFLP is highly reproducible.

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Additional file 3:

Table S4. T-RFLP profile Shannon alpha indeces.

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