This article is part of the supplement: Arthropod symbioses: from fundamental studies to pest and disease management

Open Access Research

Influence of Wolbachia on host gene expression in an obligatory symbiosis

Natacha Kremer12*, Delphine Charif1, Hélène Henri1, Frédérick Gavory3, Patrick Wincker3, Patrick Mavingui4 and Fabrice Vavre1

Author Affiliations

1 Université de Lyon, F-69000, Lyon; Université Lyon 1; CNRS, UMR5558, Laboratoire de Biométrie et Biologie Evolutive, F-69622, Villeurbanne, France

2 Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA

3 Génoscope; CEA, DSV, Institut de Génomique, F-91057, Evry, France

4 Université de Lyon, F-69000, Lyon; Université Lyon1; CNRS, VetAgro Sup, UMR 5557, Ecologie Microbienne, F-69622, Villeurbanne, France

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BMC Microbiology 2012, 12(Suppl 1):S7  doi:10.1186/1471-2180-12-S1-S7

Published: 18 January 2012

Additional files

Additional file 1:

Primers used for quantitative RT-PCR.

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Additional file 2:

Functions under-represented in wasp ovaries in response to Wolbachia infection, biological process level 6. GO terms differentially-represented in libraries from aposymbiotic (A) and symbiotic (S) ovaries (Pi3 strain). The proportion of ESTs related to each GO function is indicated in the OA library (OA1 and OA2) and in the reference library (OS). Biological processes (level 6) are sorted relative to their A/S ratio, representing the enrichment percentage in the OA library compared to the OS library. An asterisk indicates functions shared by OA1 and OA2.

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Additional file 3:

Expression profiles of genes studied in quantitative RT-PCR Quantitative RT-PCR was performed from symbiotic (gray) or aposymbiotic (white) extracts. The Pi3 strain exhibits a strong ovarian phenotype after Wolbachia removal (no eggs in the ovaries), while the NA strain produces a few eggs that do not develop normally. RNA was extracted either from 10 males or from 10 ovaries (whole ovaries for the NA strain and distal part of the ovaries for the Pi3 strain). Expression of each candidate gene was normalized by the geometric mean of three housekeeping genes. The mean of 5 biological replicates (+/- SE) is shown on the graph. *: conditions that are significantly different (Wilcoxon’s test on expression data, p-values adjusted using FDR’s correction, p-value < 0.05).

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