Characterisation of a cell wall-anchored protein of Staphylococcus saprophyticus associated with linoleic acid resistance
1 Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia
2 Department of Medical Microbiology, Institute for Hygiene and Microbiology, Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany
3 Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
4 Center for Infectious Diseases and Travel Medicine, University Medical Center Freiburg, Freiburg, Germany
5 School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK
BMC Microbiology 2012, 12:8 doi:10.1186/1471-2180-12-8Published: 15 January 2012
The Gram-positive bacterium Staphylococcus saprophyticus is the second most frequent causative agent of community-acquired urinary tract infections (UTI), accounting for up to 20% of cases. A common feature of staphylococci is colonisation of the human skin. This involves survival against innate immune defenses including antibacterial unsaturated free fatty acids such as linoleic acid which act by disrupting bacterial cell membranes. Indeed, S. saprophyticus UTI is usually preceded by perineal skin colonisation.
In this study we identified a previously undescribed 73.5 kDa cell wall-anchored protein
of S. saprophyticus, encoded on plasmid pSSAP2 of strain MS1146, which we termed
Our data indicate that SssF is a newly described and highly prevalent surface-localised protein of S. saprophyticus that contributes to resistance against the antibacterial effects of linoleic acid. SssF is a member of a protein family widely disseminated throughout the staphylococci.