Additional file 2.
Figure S2. TLC analysis of 3-oxo-C10-HSL produced byV. anguillarum. Extracted samples from V. anguillarum cultures were chromatographed on a C-18 RP-TLC plate, developed with methanol/water (70:30, v/v). The spots were visualized 13 by overlaying the TLC plate with C. violaceum VIR07. As AHL standards, Cn-HSL: 14 C6-HSL, C8-HSL and C10-HSL, 3-oxo-Cn-HSL: 3-oxo-C6-HSL, 3-oxo-C8-HSL, 15 3-oxo-C10-HSL and 3-oxo-C12-HSL were used.
Format: PDF Size: 389KB Download file
This file can be viewed with: Adobe Acrobat Reader
Minagawa et al. BMC Microbiology 2012 12:70 doi:10.1186/1471-2180-12-70