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RND type efflux pump system MexAB-OprM of pseudomonas aeruginosa selects bacterial languages, 3-oxo-acyl-homoserine lactones, for cell-to-cell communication

Shu Minagawa1, Hiroyuki Inami1, Tomohisa Kato1, Shinji Sawada1, Tatsuya Yasuki1, Shinichi Miyairi2, Manabu Horikawa3, Jun Okuda1 and Naomasa Gotoh1*

Author Affiliations

1 Department of Microbiology and Infection Control Science, Kyoto Pharmaceutical University, Yamashina, Kyoto, 607-8414, Japan

2 Laboratory of Organic Chemistry, School of Pharmacy, Nihon University, Funahashi, Chiba, 274-8555, Japan

3 Suntory Foundation for Life Science, Bioorganic Research Institute, Mishima, Osaka, 618-8503, Japan

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BMC Microbiology 2012, 12:70  doi:10.1186/1471-2180-12-70

Published: 10 May 2012

Additional files

Additional file 1:

Figure S1. Cross-streak experiment for detection of bacterial interaction via acyl-HSLs. The two monitor strains used were KG7004 (ΔlasI ΔrhlI) and KG7050 (ΔlasIΔrhlI4 ΔmexB) harboring the lasB promoter-gfp plasmid (pMQG003) were used. Test strains against the monitor strains (center) were cross-streaked on LB agar plates. Following 24 h incubation at30°C, the growth of strains was observed under a stereomicroscope, and then production of GFP by the monitor strains was visualized by excitation of the plates with blue light.

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Additional file 2:

Figure S2. TLC analysis of 3-oxo-C10-HSL produced byV. anguillarum. Extracted samples from V. anguillarum cultures were chromatographed on a C-18 RP-TLC plate, developed with methanol/water (70:30, v/v). The spots were visualized 13 by overlaying the TLC plate with C. violaceum VIR07. As AHL standards, Cn-HSL: 14 C6-HSL, C8-HSL and C10-HSL, 3-oxo-Cn-HSL: 3-oxo-C6-HSL, 3-oxo-C8-HSL, 15 3-oxo-C10-HSL and 3-oxo-C12-HSL were used.

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Additional file 3:

Supplemental information of Materials, Methods, Figure legend of Figure S1 and S2 and References[1,45-49].

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