Table 1

Primer and probe sequences of BactQuant, the new 16 S rRNA gene-based quantitative real-time PCR (bold letters denotes degenerate base)
BactQuant Tm (°C) E. coli region
Forward Primer 5′- CCTACGGGDGGC WGCA-3′ 55.9–58.4 341–356
Reverse Primer 5′- GGACTACHVGGGT MTCTAATC -3′ 57.5–63.3 786–806
Probe (6FAM) 5′-CAGCAGCCGCGGTA-3′ (MGBNFQ) 68.0 519–532

Liu et al.

Liu et al. BMC Microbiology 2012 12:56   doi:10.1186/1471-2180-12-56

Open Data