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Open Access Research article

The expression of one ankyrin pk2 allele of the WO prophage is correlated with the Wolbachia feminizing effect in isopods

Samuel Pichon12, Didier Bouchon1, Chao Liu3, Lanming Chen3, Roger A Garrett3 and Pierre Grève1*

Author Affiliations

1 Ecologie, Evolution, Symbiose, UMR CNRS 6556, Université de Poitiers, Poitiers, 86022, France

2 Zoological Institute, University of Basel, Basel, 4051, Switzerland

3 Department of Biology, University of Copenhagen, Copenhagen, 2200 N, Denmark

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BMC Microbiology 2012, 12:55  doi:10.1186/1471-2180-12-55

Published: 12 April 2012

Additional files

Additional file 1:

Figure S1. Southern blotting analyses. Reconstituted Southern blots of EcoRI or BamHI digested DNA from 8 Wolbachia-infected terrestrial isopod species hybridized with three different probes (see text for details). White triangles highlight positions of the hybridized fragments. Lanes were loaded with DNA from Wolbachia strain endosymbionts of PDP as P. dilatatus petiti; PDD as P. dilatatus dilatatus; CC as C. convexus; AVC as A. vulgare strain wVulC; AVM as A. vulgare strain wVulM; AN as A. nasatum; OA as O. asellus; PP as P. pruinosus strain wPruIII. The number of bands in some lanes is higher than the number of copies presented in Table 2 due to EcoRI and/or BamHI restriction site(s) in these copies, as confirmed by sequencing. Upper light bands correspond to partially digested DNA fragments. Figure S2. Phylogenetic tree ofWolbachiastrains based on thewspgene.Wolbachia strains of isopods are shown in bold (wAlbum: Armadillidium album; wAse: Oniscus asellus; wConV: Cylisticus convexus; wDil: Porcellio dilatatus dilatatus; wElo: Chaetophiloscia elongata; wHoo: Sphaeroma hookeri; wMus: Philoscia muscorum; wNas: Armadillidium nasatum; wOce: Ligia oceanica; wPet: Porcellio dilatatus petiti; wPruIII: Porcellionides pruinosus; wRug: Sphaeroma rugicauda; wScaber: Porcellio scaber; wVulC, wVulM, wVulP: Armadillidium vulgare). The additional B-supergroup Wolbachia strains and the host phenotypes they induce are based on previously published information (wAlbB: Aedes albopictus; wAlt: Chelymorpha alternans; wAu, wMa, wNo, wRi: Drosophila simulans; wBol: Hypolimnas bolina; wCauB: Cadra cautella; wCon: Tribolium confusum; wDei: Trichogramma deion; wEnc: Acraea encedon; wFor: Encarsia formosa; wFir: Gryllus firmus; wKue: Ephestia kuehniella; wMel: Drosophila melanogaster; wOri: Tagosodes orizicolus; wPip-JHB, wPip-Pel: Culex pipiens quinquefasciatus; wScap: Ostrinia scapulalis; wSn: Drosophila sechellia; wStri: Laodelphax striatellus; wTai: Teleogryllus taiwanemma; wVitA: Nasonia vitripennis). Confirmed or suspected induced-phenotypes of Wolbachia strains of isopods are drawn from Bouchon et al. (2008). The red colour of strains corresponds to the feminizing induced-phenotype, blue to CI, green to male killing, light grey to parthenogenesis, and black to suspected feminization. Node supports are shown by posterior probabilities from Bayesian inferences. Figure S3. SMART outputs representing the number of ANK motifs found in Pk1 translated sequences. Figure S4. SMART outputs representing the number of ANK motifs found in Pk2 translated sequences. Table S1. List of primers used in this study for sequencing (PCR), for expression analyses (RT-PCR), or for Southern blots (SB). Expected PCR product size in base pair (bp) was calculated relative to the wVulC reference sequences. Table S2. List ofpk1andpk2sequences used for Figure 1, Additional file 1: Figure S3 and Additional file 1: Figure S4. Accession numbers from this study are in bold.

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