Open Access Research article

Identification and diversity of multiresistant Corynebacterium striatum clinical isolates by MALDI-TOF mass spectrometry and by a multigene sequencing approach

Margarita Gomila1*, Feliu Renom2, Maria del Carmen Gallegos3, Margarita Garau3, Dolores Guerrero4, Joan B Soriano4 and Jorge Lalucat5

Author Affiliations

1 Unidad de Investigación-Microbiología, Fundación Hospital Son Llàtzer, Ctra. Manacor, km. 4, 07198 Palma de Mallorca, Illes Balears, Spain

2 Respiratory Unit, Hospital Joan March, Ctra. Sóller, km. 12, 07110 Bunyola, Illes Balears, Spain

3 Microbiology Service, Fundación Hospital Son Llàtzer, Ctra. Manacor, km. 4, 07198 Palma de Mallorca, Illes Balears, Spain

4 Program of Epidemiology and Clinical Research, Fundació Caubet-CIMERA Illes Balears, International Centre for Advanced Respiratory Medicine, Ctra. Sóller, km. 12, 07110 Bunyola, Illes Balears, Spain

5 Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), Ctra. Valldemossa, km. 7.5, 07122 Palma de Mallorca, Illes Balears, Spain

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BMC Microbiology 2012, 12:52  doi:10.1186/1471-2180-12-52

Published: 4 April 2012

Additional files

Additional file 1:

Table S1. List of isolates analysed, their origin and sample type. External strains for comparison purposes have been included in the study: the type strains C. amycolatum CCUG 35685T and C. striatum ATCC 6940T, as well as two strains of C. striatum with different origins, CCUG 39137 (from a human wound) and CCUG 44705 (tobacco industry).

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Additional file 2:

Table S2. Primers used for performing the molecular analysis of the 56 Corynebacterium strains.

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Additional file 3:

Table S3. Phenotypic results of RapID CB Plus® tests for the different strains analysed.

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Additional file 4:

Table S4. Antibiotic susceptibility pattern of each strain analysed. The antibiotics tested for all strains were penicillin (PEN), imipenem (IMI), erythromycin (ERI), rifampicin (RIF), tetracycline (TET), vancomycin (VAN), ciprofloxacin (CIP), gentamicin (GEN), cefotaxime (CEF), and trimethoprim-sulfamethoxazole (TRI). R, resistant; I, intermediate; S, susceptible.

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Additional file 5:

Figure S1. ERIC-PCR patterns of the different C. striatum clinical isolates analysed. The number on the top of the lane corresponds to the number of clinical isolate studied; CsT, C. striatum ATCC 6940T. M1, Marker λE/H; M2, marker 100 bp.

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Additional file 6:

Figure S2. SARAMIS cluster analysis of all Corynebacterium strains isolated.

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