Table 1

Lifespan and intestinal colonization of C.elegans N2 and mutants with growth on E. colior Salmonellalawnsa

E. coli OP50

S. typhimuriumSL1344


Genotype

Symbol

TD50

(Mean ± SD)

Day 2 log10 intestinal cfu (Mean ± SD)

TD50

(Mean ± SD)

Day 2 log10 intestinal cfu (Mean ± SD)


N2

12.93 ± 0.50

2.76 ± 0.22

10.87 ± 1.37

3.22 ± 0.07

daf-2

26.45 ± 1.34^^

1.70 ± 0.12^^

20.17 ± 0.29^^

1.87 ± 0.15^^

age-1

18.75 ± 0.35^^

2.48 ± 0.32

13.70 ± 0.14^

2.36 ± 0.48^

daf-16

8.05 ± 0.38^^

3.30 ± 0.19

5.53 ± 0.23^^

3.55 ± 0.15^

lys-7

9.30 ± 0.74^

2.93 ± 0.39

8.83 ± 0.25^

3.31 ± 0.28

spp-1

9.80 ± 0.59^

2.67 ± 0.27

8.70 ± 0.14^

3.41 ± 0.23

sod-3

11.90 ± 1.01

2.87 ± 0.24

10.93 ± 1.23

3.45 ± 0.25

ctl-2

9.48 ± 0.29^

2.69 ± 0.18

8.98 ± 0.67^

3.88 ± 0.14^

dbl-1

5.80 ± 0.57^^

3.35 ± 0.06

4.75 ± 0.79^^

3.86 ± 0.19^

lys-1

10.00 ± 0.40^

2.60 ± 0.22

8.95 ± 0.44^

3.12 ± 0.24

pmk-1

7.40 ± 0.16^^

2.58 ± 0.34

6.10 ± 0.99^^

3.71 ± 0.78^

tol-1

10.53 ± 0.31^^

2.81 ± 0.15

8.98 ± 0.79^

3.53 ± 0.18^

trx-1

7.70 ± 0.14^^

2.95 ± 0.17

6.83 ± 0.38^^

3.30 ± 0.38


a Worms were age-synchronized by a bleaching procedure. Embryos were placed on mNGM agar plates containing E. coli OP50 or S. typhimurium SL1344 and incubated at 25°C. The L4 stage was designated as day 0. A total of 100 worms were used per lifespan assay. Bacterial colonization of the intestinal tract was determined at day 2 by washing and grinding 10 worms, and plating worm lysates on MacConkey agar. All assays were performed at least three times

^p< 0.05, compared to N2

^^p< 0.001, compared to N2

Portal-Celhay et al. BMC Microbiology 2012 12:49   doi:10.1186/1471-2180-12-49

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