16S rRNA-RFLP patterns (agarose gel 3.5%) obtained for Arcobacter spp. using the endonuclease MseI. Lanes: L, 50 bp ladder, Fermentas. The obtained patterns agree with those expected from the computer simulation (Additional file 1: Table S1). Species that share an identical or similar pattern (Additional file 1: Table S1) were: A. butzleri, that produced a pattern identical to those of A. trophiarum, A. thereius and atypical strains (n=11) of A. cryaerophilus; A. marinus CECT 7727T with a pattern very similar to the one of A. venerupis CECT 7836T and A. defluvii with an identical pattern to the one of A. suis strain F41. The identification of these species required additional digestions with other enzymes (Figures 2 – 4, Additional file 2: Table S2 and Additional file 3: Table S3).
Figueras et al. BMC Microbiology 2012 12:292 doi:10.1186/1471-2180-12-292