Table 1 |
|||||||
| B. pseudomalleiP2-like prophage distribution screen | |||||||
| P2-like prophage PCR probe results | |||||||
| Multiplex | |||||||
| B. pseudomallei | Candidate P2-like prophage group a | P2-like 1 | P2-like 2 | P2-like group A | φX216 scrnA | φX216 scrnB | GI2 |
| Strains with high φX216 plaquing efficiency b 66.7% (22/33) P2-like prophage candidate positive strains | |||||||
| 2668a | φ52237-like | + | + | + | + | + | + |
| E0237 c | φ52237-like | + | + | + | + | + | + |
| E0394 | φ52237-like | + | + | + | + | + | + |
| 1026b d | φ52237-like | + | + | + | + | + | + |
| 708a | φ52237-like e | + | + | + | + | - | + |
| 2618a | P2L-A | + | + | + | - | - | + |
| 2661a | P2L-A | + | + | + | - | - | + |
| 2692a | P2L-A | + | + | + | - | - | + |
| 2717a | P2L-A | + | + | + | - | - | + |
| E0021 | P2L-A | + | + | + | - | - | + |
| E0235 | P2L-A | + | + | + | - | - | + |
| E0279 | P2L-A | + | + | + | - | - | + |
| E0345 | P2L-A | + | + | + | - | - | + |
| E0384 | P2L-A | + | + | + | - | - | + |
| E0386 | P2L-A | + | + | + | - | - | + |
| K96243 f | P2L-A | + | + | + | - | - | + |
| S13 g | P2L-A | + | + | + | - | - | + |
| 2698a | P2L | + | + | - | - | - | - |
| 2704a | P2L h | + | + | - | - | + | - |
| E0342 | P2L | + | + | - | - | - | - |
| E0366 | P2L | + | + | - | - | - | - |
| E0377 | P2L | + | + | - | - | - | - |
| 2613a | - | - | - | - | - | ND i | |
| 2667a | - | - | - | - | - | ND | |
| 2673a | - | - | - | - | - | ND | |
| 2682a | - | - | - | - | - | ND | |
| 2769a | - | - | - | - | - | ND | |
| E0016 | - | - | - | - | - | ND | |
| E0034 | - | - | - | - | - | ND | |
| E0241 | - | - | - | - | - | ND | |
| E0356 | - | - | - | - | - | ND | |
| E0411 | - | - | - | - | - | ND | |
| MSHR305 | - | - | - | - | - | ND | |
| Strains with low φX216 plaquing efficiency j 17.4% (4/23), P2-like prophage candidate positive strains | |||||||
| 2625a | φ52237-like | + | + | + | + | + | + |
| 2670a | P2L-A | + | + | + | - | - | + |
| E0037 | P2L-A | + | + | + | - | - | + |
| E0380 | P2L-A | + | + | + | - | - | + |
| 2637a | - | - | - | - | - | ND | |
| 2650a | - | - | - | - | - | ND | |
| 2660a | - | - | - | - | - | ND | |
| 2685a | - | - | - | - | - | ND | |
| 2708a | - | - | - | - | - | ND | |
| 2719a | - | - | - | - | - | ND | |
| 2764b | - | - | - | - | - | ND | |
| E0024 | - | - | - | - | - | ND | |
| E0031 | - | - | - | - | - | ND | |
| E0181 | - | - | - | - | - | ND | |
| E0371 | - | - | - | - | - | ND | |
| E0372 | - | - | - | - | - | ND | |
| E0378 | - | - | - | - | - | ND | |
| E0383 | - | - | - | - | - | ND | |
| E0393 | - | - | - | - | - | ND | |
| 1710a | - | - | - | - | - | ND | |
| 1710b k | - | - | - | - | - | - | |
| 1106b | - | - | - | - | - | ND | |
| 406e | - | - | - | - | - | ND | |
| Non-φX216 plaquing strains 25.0% (4/16), P2-like prophage candidate positive strains | |||||||
| 2671a | P2L-A | + | + | + | - | - | + |
| 2674a | P2L-A | + | + | + | - | - | + |
| 2677a | P2L-A | + | + | + | - | - | + |
| Pasteur 6068 | P2L-A | + | + | + | - | - | + |
| 2614a | - | - | - | - | - | ND | |
| 2617a | - | - | - | - | - | ND | |
| 2640a | - | - | - | - | - | ND | |
| 2665a | - | - | - | - | - | ND | |
| 2689b | - | - | - | - | - | ND | |
| 2694a | - | - | - | - | - | ND | |
| E0008 | - | - | - | - | - | ND | |
| E0183 | - | - | - | - | - | ND | |
| E0350 | - | - | - | - | - | ND | |
| E0396 | - | - | - | - | - | ND | |
| 1106a | - | - | - | - | - | ND | |
| MSHR668 | - | - | - | - | - | ND | |
a φ52237-like assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2, P2-like group A, and individual PCR probes φX216 scrnA, φX216 scrnB and GI2. P2L-A assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2, P2-like group A and individual PCR probe GI2. P2L assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2.
bConfluent lysis when spot tested with ~106 pfu φX216.
cφX216 source strain.
d1026b φ52237-like prophage is split into two segments and likely non-functional [15].
eP2-like prophage group cannot be determined based on PCR results. May be P2L-A or φ52237-like.
fφK96243 prophage (group P2-A) located at GI2 [9].
gEncodes the predicted prophage PI-S13-1 (group P2-A) [88].
hP2-like prophage group cannot be assigned based on PCR results. May be P2L or φ52237-like.
IND, GI2 probe results not determined.
jNon-confluent lysis / individual plaques when spot tested with ~ 106 pfu φX216.
kThe strain 1710b genome does not contain a P2-like prophage or prophage insertion at GI2.
Kvitko et al. BMC Microbiology 2012 12:289 doi:10.1186/1471-2180-12-289
