Table 1

B. pseudomalleiP2-like prophage distribution screen
P2-like prophage PCR probe results
Multiplex
B. pseudomallei Candidate P2-like prophage group a P2-like 1 P2-like 2 P2-like group A φX216 scrnA φX216 scrnB GI2
Strains with high φX216 plaquing efficiency b 66.7% (22/33) P2-like prophage candidate positive strains
2668a φ52237-like + + + + + +
E0237 c φ52237-like + + + + + +
E0394 φ52237-like + + + + + +
1026b d φ52237-like + + + + + +
708a φ52237-like e + + + + - +
2618a P2L-A + + + - - +
2661a P2L-A + + + - - +
2692a P2L-A + + + - - +
2717a P2L-A + + + - - +
E0021 P2L-A + + + - - +
E0235 P2L-A + + + - - +
E0279 P2L-A + + + - - +
E0345 P2L-A + + + - - +
E0384 P2L-A + + + - - +
E0386 P2L-A + + + - - +
K96243 f P2L-A + + + - - +
S13 g P2L-A + + + - - +
2698a P2L + + - - - -
2704a P2L h + + - - + -
E0342 P2L + + - - - -
E0366 P2L + + - - - -
E0377 P2L + + - - - -
2613a - - - - - ND i
2667a - - - - - ND
2673a - - - - - ND
2682a - - - - - ND
2769a - - - - - ND
E0016 - - - - - ND
E0034 - - - - - ND
E0241 - - - - - ND
E0356 - - - - - ND
E0411 - - - - - ND
MSHR305 - - - - - ND
Strains with low φX216 plaquing efficiency j 17.4% (4/23), P2-like prophage candidate positive strains
2625a φ52237-like + + + + + +
2670a P2L-A + + + - - +
E0037 P2L-A + + + - - +
E0380 P2L-A + + + - - +
2637a - - - - - ND
2650a - - - - - ND
2660a - - - - - ND
2685a - - - - - ND
2708a - - - - - ND
2719a - - - - - ND
2764b - - - - - ND
E0024 - - - - - ND
E0031 - - - - - ND
E0181 - - - - - ND
E0371 - - - - - ND
E0372 - - - - - ND
E0378 - - - - - ND
E0383 - - - - - ND
E0393 - - - - - ND
1710a - - - - - ND
1710b k - - - - - -
1106b - - - - - ND
406e - - - - - ND
Non-φX216 plaquing strains 25.0% (4/16), P2-like prophage candidate positive strains
2671a P2L-A + + + - - +
2674a P2L-A + + + - - +
2677a P2L-A + + + - - +
Pasteur 6068 P2L-A + + + - - +
2614a - - - - - ND
2617a - - - - - ND
2640a - - - - - ND
2665a - - - - - ND
2689b - - - - - ND
2694a - - - - - ND
E0008 - - - - - ND
E0183 - - - - - ND
E0350 - - - - - ND
E0396 - - - - - ND
1106a - - - - - ND
MSHR668 - - - - - ND

a φ52237-like assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2, P2-like group A, and individual PCR probes φX216 scrnA, φX216 scrnB and GI2. P2L-A assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2, P2-like group A and individual PCR probe GI2. P2L assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2.

bConfluent lysis when spot tested with ~106 pfu φX216.

cφX216 source strain.

d1026b φ52237-like prophage is split into two segments and likely non-functional [15].

eP2-like prophage group cannot be determined based on PCR results. May be P2L-A or φ52237-like.

fφK96243 prophage (group P2-A) located at GI2 [9].

gEncodes the predicted prophage PI-S13-1 (group P2-A) [88].

hP2-like prophage group cannot be assigned based on PCR results. May be P2L or φ52237-like.

IND, GI2 probe results not determined.

jNon-confluent lysis / individual plaques when spot tested with ~ 106 pfu φX216.

kThe strain 1710b genome does not contain a P2-like prophage or prophage insertion at GI2.

Kvitko et al.

Kvitko et al. BMC Microbiology 2012 12:289   doi:10.1186/1471-2180-12-289

Open Data