Multiplex PCR for detection of φX216-related P2-like prophage in B. pseudomallei strains. Genomic DNA preparations of B. pseudomallei strains were used as PCR templates in multiplex PCR. Upper and lower fragments only (B. pseudomallei 2698a and 2704a) indicates presence of a P2-like (P2L) prophage. The presence of three fragments (B. pseudomallei 2692a and 2717a) indicates presence of a P2-like subgroup A prophage (P2L-A). The three marked DNA fragments correspond (top-to-bottom) to the fels-2 PCR product (418 bp), the int gene PCR product (316 bp), and the capsid gene N PCR product (248 bp). Lanes M, Hi-Lo molecular size ladder from Minnesota Molecular (Minneapolis, MN).
Kvitko et al. BMC Microbiology 2012 12:289 doi:10.1186/1471-2180-12-289