Table 1

Strains and plasmids used in this study
Strain or plasmid Description Reference or source
RN6390B Standard laboratory strain NARSAa
RN4220 8325-4 r- NARSA
ΔluxS RN6390B luxS::ermB This study
RN6911 RN6390B derivative; agr locus replaced with tetM cassette NARSA
ΔagrΔluxS RN6911 luxS::ermB, agr/luxS double mutant This study
ΔluxSpluxS Complemented strain of ΔluxS; Apr Cmr This study
RN6390BG RN6390B/pgfp This study
ΔluxSG ΔluxS/pgfp This study
RN6911G RN6911/pgfp This study
ΔagrΔluxSG ΔagrΔluxS/pgfp This study
NCTC8325 Standard Laboratory strain NARSA
NCTC8325ΔluxS NCTC8325 luxS::ermB 60
E. coli strains
TOP10 Cloning Invitrogen
Plasmids
pEASY-Blunt Clone vector, Kanr Apr Transgen
pBTluxS Vector used for luxS mutagenesis, Apr Cmr Emr 60
pLI50 E. coli-S. aureus shuttle cloning vector, Apr Cmr Addgene
pLIluxS pLI50 with luxS and its promoter, Apr Cmr 60
pgfp gfp expression with the promoter of S10 ribosomal gene, Apr, Cmr

aNARSA, Network on Antimicrobial Resistance in Staphylococcus aureus.

Yu et al.

Yu et al. BMC Microbiology 2012 12:288   doi:10.1186/1471-2180-12-288

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