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Open Access Highly Accessed Research article

Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner

Dan Yu, Liping Zhao, Ting Xue and Baolin Sun*

Author Affiliations

Department of Microbiology and Immunology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230027, China

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BMC Microbiology 2012, 12:288  doi:10.1186/1471-2180-12-288

Published: 5 December 2012

Additional files

Additional file 1:

Relative transcript levels of several adhesions. The levels of transcription of these genes including map, fnbA, fnbB, clfB, efb were measured by real-time RT-PCR in S. aureus WTp, ΔluxSp and ΔluxS complemented with a plasmid containing luxS gene for genetic complementation (ΔluxSpluxS). As the control, WT and ΔluxS were transformed with empty plasmid PLI50, constructing WTp and ΔluxSp.

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Additional file 2:

Extracellular protein loaded on SDS-PAGE. The levels of extracellular-protein expression of biofilm bacteria, which were incubated at 37°C for 4 h and 24 h, were measured.

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Additional file 3:

Triton X-100-stimulated autolysis. The autolysis of WT, ΔluxS and ΔluxSpluxS induced in 0.05 M Tris–HCl buffer containing 0.05% (vol/vol) Triton X-100 were measured.

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