Table 1

Comparative proteome profile of P. putida grown at 50 rpm and 150 rpm
Locus tag Protein name Accession number Fold-change Protein function
Up-regulated proteins (50 rpm/150 rpm)
PP_0234 OprE gi|26986977 2.41* Outer membrane porin
PP_0268 OprQ gi|26987010 1.80 Outer membrane porin
PP_0465 RplX gi|26987206 1.61 50S ribosomal protein L24
PP_0812 CyoA gi|26987548 1.82 Ubiquinol oxidase subunit 2
PP_0988 GcvP-1 gi|26987724 2.53 Glycine dehydrogenase
PP_1037 PurL gi|26987773 1.59* Phosphoribosylformylglycinamidine synthase
PP_1099 gi|26987835 1.74 Cold-shock domain-contain protein
PP_1629 RecA gi|26988361 2.35* Recombinase A
PP_1868 gi|26988598 2.25* DEAD-box ATP dependent DNA helicase
PP_1982 IbpA gi|26988708 8.33* Heat shock protein Hsp20
PP_2468 RplT gi|26989191 1.64 50S ribosomal protein L20
PP_2645 MgtB gi|26989364 2.67* Magnesium-translocating P-type ATPase
PP_2656 PstS gi|26989375 4.26* Phosphate ABC transporter, periplasmic phosphate-binding protein
PP_4718 FtsH gi|26991401 2.04 ATP-dependent metalloprotease FtsH
PP_4803 DacA gi|26991483 1.96* Serine-type D-Ala-D-Ala carboxypeptidase
PP_5329 PstS gi|26992005 3.33* Phosphate ABC transporter phosphate-binding protein
PP_0460 gi|24981839 1.65 Ribosomal protein S3
Down-regulated proteins (50 rpm/150 rpm)
PP_0126 gi|26986871 0.37* Cytochrome c4
PP_0258 gi|26987000 0.21* Hypothetical protein PP_0258
PP_0296 gi|26987038 0.36* Glycine betaine/L-proline ABC transporter, periplasmic binding protein
PP_0308 gi|26987050 0.37 Membrane dipeptidase
PP_0315 gi|26987057 0.22 Rieske (2Fe-2S) domain protein
PP_0322 GlyA-1 gi|26987064 0.44 Serine hydroxymethyltransferase
PP_0328 FdhA gi|26987070 0.38* Formaldehyde dehydrogenase, glutathione-independent
PP_0382 gi|26987124 0.41 Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase
PP_0395 gi|26987137 0.19 Hypothetical protein PP_0395
PP_0397 gi|26987139 0.28* Putative serine protein kinase, PrkA
PP_0541 gi|26987279 0.28 Acetyltransferase
PP_0545 gi|26987283 0.43* Aldehyde dehydrogenase family protein
PP_0763 gi|26987499 0.50 Acyl-CoA synthetase
PP_0765 gi|26987501 0.45* Hypothetical protein PP_0765
PP_0951 RpoX gi|26987687 0.34* Sigma 54 modulation protein/ribosomal protein S30EA
PP_0999 ArcC gi|26987735 0.23* Carbamate kinase
PP_1000 ArgI gi|26987736 0.28* Ornithine carbamoyltransferase
PP_1001 ArcA gi|26987737 0.24* Arginine deiminase
PP_1015 gi|26987751 0.52 Sugar ABC transporter, periplasmic sugar-binding protein
PP_1081 gi|26987817 0.44* Glutaredoxin-related protein
PP_1084 gi|26987820 0.42 Anti-oxidant AhpCTSA family protein
PP_1122 gi|26987858 0.22 OmpA/MotB domain protein
PP_1210 gi|26987945 0.32* DNA-binding stress protein, putative
PP_1478 gi|26988211 0.23* NADH:flavin oxidoreductase/NADH oxidase
PP_1487 gi|26988220 0.40* Hypothetical protein PP_1487
PP_1506 Adk gi|26988238 0.34* Adenylate kinase
PP_1661 gi|26988393 0.41* Dehydrogenase subunit, putative
PP_1741 gi|26988472 0.28* Substrate-binding region of ABC-type glycine betaine transport system
PP_1859 Ohr gi|26988589 0.16* OsmC family protein
PP_2006 gi|26988731 0.12* Hypothetical protein PP_2006
PP_2105 gi|26988830 0.48 Hypothetical protein PP_2105
PP_2112 AcnA gi|26988836 0.42* Aconitate hydratase
PP_2140 gi|26988864 0.47 Hypothetical protein PP_2140
PP_2303 HupB gi|26989027 0.52 Histone family protein DNA-binding protein
PP_3089 gi|26989808 0.37* Hypothetical protein PP_3089
PP_3232 gi|26989950 0.16* Acetyltransferase
PP_3283 PhaB gi|26990001 0.21* Enoyl-CoA hydratase
PP_3433 Hpd gi|26990146 0.25* 4-hydroxyphenylpyruvate dioxygenase
PP_3611 gi|26990322 0.12* Hypothetical protein PP_3611
PP_3668 gi|26990379 0.28* Catalase/peroxidase HPI
PP_3765 gi|26990470 0.24* Transcriptional regulator MvaT, P16 subunit, putative
PP_3839 AdhA gi|26990544 0.30* Alcohol dehydrogenase
PP_4011 Icd gi|26990716 0.25* Isocitrate dehydrogenase, NADP-dependent
PP_4034 gi|26990737 0.38* Allantoate amidohydrolase
PP_4037 gi|26990739 0.32* Putative oxidoreductase
PP_4038 gi|26990740 0.26* Dihydropyrimidine dehydrogenase
PP_4116 AceA gi|26990810 0.27* Isocitrate lyase
PP_4486 gi|26991172 0.51 Cationic amino acid ABC transporter, periplasmic binding protein
PP_4490 PhhA gi|26991176 0.47* Phenylalanine 4-monooxygenase
PP_4593 gi|26991277 0.20* Hypothetical protein PP_4593
PP_4666 MmsB gi|26991350 0.24* 3-hydroxyisobutyrate dehydrogenase
PP_4667 MmsA-2 gi|26991351 0.28* Methylmalonate-semialdehyde dehydrogenase
PP_4848 gi|26991528 0.54 DnaJ family curved-DNA-binding protein
PP_4870 gi|26991550 0.38* Azurin
PP_5007 gi|26991684 0.33* Poly(hydroxyalkanoate) granule-associated protein
PP_5220 ElbB gi|26991896 0.45 Isoprenoid biosynthesis protein
PP_5232 gi|26991908 0.48 Hypothetical protein PP_5232
PP_5258 gi|26991934 0.27* Aldehyde dehydrogenase family protein
PP_5260 gi|26991936 0.24* Hypothetical protein PP_5260

* P-value < 0.05.

Crabbé et al.

CrabbĂ© et al. BMC Microbiology 2012 12:282   doi:10.1186/1471-2180-12-282

Open Data