Additional file 4: Figure S3.

pgaA mRNA decay analysis. Bacterial cultures of C-1a (pnp+), C-5691 (Δpnp) and C-5938 (ΔcsrA) were grown up to OD600 = 0.8 in M9Glu/sup, rifampicin (final concentration of 0.4 mg/ml) was added, and samples for RNA extraction were taken at different time points immediately before (t = 0) and after antibiotic addition. pgaA mRNA degradation kinetics was estimated by quantitative RT-PCR with oligonucleotides PL99 and PL100, as detailed in Methods.

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Carzaniga et al. BMC Microbiology 2012 12:270   doi:10.1186/1471-2180-12-270