Additional file 4.

Figure S1. Nucleotide sequences of the predicted ctRNA coding strands. The counter-transcripts were first identified by analogy with those of pMV158 or its derivative pLS1. These ctRNA overlap the rep gene start and have a length of only a few tens of nucleotides. Using the consensus sequence TTGACA – (N17) –TG-N-TATAAT for the promoter, putative promoters were identified in the aligned sequences. Putative Pct promoters are indicated with the -35 and -10 regions in bold and underlined letters. Arrows indicate inverted repeats of the putative rho independent terminators. The ctRNA of pLS1 (rnaII) is shown as proposed by del Solar et al. [46] with an arrowhead indicating the possible transcriptional initiation site. The box CAT indicates the initiation codon of the rep gene that is encoded on the complementary DNA strand.

Format: DOCX Size: 37KB Download file

Breton et al. BMC Microbiology 2012 12:257   doi:10.1186/1471-2180-12-257