Table 1

Strains and plasmids used in this study
Strain and plasmid Genotype or description Source or reference
Strains
Streptomyces strains (Y27, Y32, Y33, Y34, Y41, Y42 and G2-1) Isolated from Gingko harboring pWTY27 This work
Streptomyces strains(W15, W24, W37 and W41) Isolated from Artemisia annua L harboring pWTY27 This work
Streptomyces strains (Z20, Z54 and Z70) Isolated from Taxus harboring pWTY27 This work
S. lividans ZX7 pro-2 str-6 rec-46 dnd SLP2- SLP3- 34
S. coelicolor A3(2) SCP1 SCP2 35
Escherichia coli DH5α F- deoR recA1 endA1 hsdR17(rk- mk+) phoA supE44 λ- thi-1 gyrA96 relA1 Invitrogen
E. coli BL21 (DE3) hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) Novagen
E. coli ET12567 (pUZ8002) dam dcm hsdM cm kan 35
Plasmids
pSP72 amp colEI ori Invitrogen
pIJ702 tsr melC pIJ101 ori 39
pYQ1 A 14-kb SacI-fragment cloned in pSP72 This work
pQC156 A 2.6-kb BclI-fragment of melC/tsr cloned in pSP72 (BglII) 26
pZR131 Two 381-bp telomeres tsr melC amp colEI ori 8
pWT177 A 3.8-kb fragment (100-3941 bp) cloned in pZR131 (EcoRI) This work
pSET152 amp apr oriT int(phiC31) 36
pWT181 pSET152 derivative, amp tsr melC cos oriT int(phiC31) This work
pET28b kan Novagen
pWT111 A 1.6-kb fragment (574-2253 bp of pWTY27) cloned in pET28b (EcoRI+HindIII) This work
pWT371 A 1.7-kb fragment (8124-9836 bp of pWTY27) cloned in pET28b (NheI+HindIII) This work
pFX144 A 1.3-kb fragment (37-1328 bp of pIJ773 containing oriT/apr) cloned in pSP72 (XbaI) This work
pWT26 A 1.3-kb fragment (13-1369 bp of pFX144 containing oriT/apr) cloned in pYQ1(EcoRV) This work
pWT24 A 5.4-kb fragment (13942-14288/1-5114 bp of pWTY27) cloned in pFX144 (SspI + SacI) This work
pWT147 A 3.8-kb fragment (100-3941 bp) cloned in pFX144 (XbaI) This work
pWT219 A 3.2-kb fragment (321-3506 bp) cloned in pFX144 (XbaI) This work
pWT217 A 1.9-kb fragment (321-2267 bp) cloned in pFX144 (XbaI) This work
pWT222 A 2.9-kb fragment (621-3506 bp) cloned in pFX144 (XbaI) This work
pWT223 A 0.3-kb fragment (321-620 bp) containing iteron cloned in pWT222 (BamHI) This work
pWT241 A 0.15-kb fragment (382-530 bp) containing iteron cloned in pWT224 (BamHI) This work
pWT34 A 95-bp fragment (1073-1167 bp) deleted from pWT24 This work
pWT33 A 259-bp fragment (2433-2691 bp) deleted from pWT24 This work
pWT203 A 6-kb fragment containing the rep/rlrA/rorA of pSLA2 cloned in pFX144 (PvuII) This work
pWT208 A 3.2-kb fragment (6757-9977 bp) cloned in pWT203 (SspI) This work
pWT207 A 1.5-kb fragment (6757-8270 bp) cloned in pWT203 (SspI) This work
pWT210 A 2.2-kb fragment (7734-9977 bp) cloned in pWT203 (SspI) This work
pWT225 A 2.2-kb fragment (7734-9893 bp) cloned in This work
pWT224 pWT203 (SspI) This work
A 2.1-kb fragment (7734-9818 bp) cloned in pWT203 (SspI)
pWT242 A 175-bp fragment (9803-9977 bp) cloned in This work
pWT224 (SspI)
pWT262 A 46-bp fragment (9803-9848 bp) cloned in This work
pWT224 (SspI)
pWT231 A 87-bp fragment (9803-9889 bp) cloned in This work
pWT224 (SspI)
pWT229 A 100-bp fragment (9803-9902 bp) cloned in This work
pWT224 (SspI)
pWT239 A 128-bp fragment (9803-9930 bp) cloned in This work
pWT224 (SspI)
pWT238 A 150-bp fragment (9803-9952 bp) cloned in This work
pWT224 (SspI)
pWT251 A 134-bp fragment (9844-9977 bp) cloned in This work
pWT224 (SspI)
pWT259 A 165-bp fragment (9813-9977 bp) cloned in This work
pWT224 (SspI)
pWT265 A 159-bp fragment (9819-9977 bp) cloned in This work
pWT224 (SspI)

Wang et al.

Wang et al. BMC Microbiology 2012 12:253   doi:10.1186/1471-2180-12-253

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