Characterization of the binding reaction of TraA protein withcltDNA by EMSA and footprinting. (a). Characteristics of a clt sequence on pWTY27 for plasmid transfer. Possible DC (direct repeat) and IC (inverted repeat) sequences are shown. (b) as Figure 2 (b). (c) as Figure 2 (c). The amounts of TraA protein used in lanes 1–5 were 0, 0.6, 1.4, 2.8 and 4.2 μg, respectively. Two sequences protected by TraA from digestion with DNaseI are shown.
Wang et al. BMC Microbiology 2012 12:253 doi:10.1186/1471-2180-12-253