|Production of biogenic amines in presence of epithelial cells|
|Precursors added||Bacteria +Human cells||Bacteria||Human cells|
|Put (μM)||Tym (μM)||Put (μM)||Tym||Put (μM)||Tym(μM)|
|Agm (4.3 mM)||1980±170a||ND||190±80c||ND||10±2d||ND|
|Tyr (10 mM)||ND||180±9a||ND||220±1ab||ND||ND|
|Tyr (10 mM) + Agm (4.3 mM)||1330±420a||230±9ab||1003±41b||320±80b||7±0d||ND|
Tyramine (Tym) and putrescine (Put) were detected by RP-HPLC in samples containing DMEM medium supplemented or not with 10 mM tyrosine, 4.38 mM agmatine or both precursors, after 8 h incubation. Cells present during the assay: Bacteria + Human cells: L. brevis IOEB 9809 (108 CFU mL-1) and Caco-2 cells (105 cells mL-1); Bacteria: L. brevis IOEB 9809 (108 CFU mL-1) and Human cells: Caco-2 cells (105 cells mL-1). Results are expressed as the mean ± standard deviation of three independent experiments. ND: not detected. Detection limits: for Put > 2 nM and for Tym > 2.5 nM. Putrescine and tyramine were below the detection limits in the DMEM medium as well as in samples containing either bacteria or Caco-2 cells in absence of the corresponding BA precursor. Differences were assessed by Anova test. Different superscript letters associated with values of the same BA indicate statistically significant differences (P < 0.05).
Russo et al.
Russo et al. BMC Microbiology 2012 12:247 doi:10.1186/1471-2180-12-247