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Open Access Research article

Staphylococcus aureus and Escherichia coli have disparate dependences on KsgA for growth and ribosome biogenesis

Heather C O’Farrell1 and Jason P Rife12*

Author Affiliations

1 Department of Physiology and Molecular Biophysics, Virginia Commonwealth University, Richmond, VA, USA

2 Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, VA, USA

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BMC Microbiology 2012, 12:244  doi:10.1186/1471-2180-12-244

Published: 24 October 2012

Additional files

Additional file 1:

Growth curves of RN and ΔksgA strains. Data represent experiments performed in triplicate; error bars indicate standard deviation.

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Open Data

Additional file 2:

Growth curves of pCN constructs. Data represent experiments performed in triplicate; error bars indicate standard deviation.

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Open Data

Additional file 3:

Primers used in knockout construction, KsgA cloning, and mutagenesis.

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Additional file 4:

Antibiotic resistance of RN4220, ΔksgA, and ΔksgA + pCN51-KsgA strains.

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Open Data

Additional file 5:

Activity assay. Experiments were performed in triplicate; error bars indicate standard deviation.

Format: PDF Size: 25KB Download file

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Open Data