Isolation of oligogalacturonides (OGAs) with varying degree of polymerization. As the chromatogram of C. annuum cell wall material co-incubated with an X. campestris pv. campestris culture was identical to OGAs derived from pectin digested with a pectate lyase, the products of the co-incubation were assumed to be OGAs, too. The activity of the X. campestris pv. campestris culture supernatant had obviously generated a diverse set of OGAs varying by their degree of polymerization (DP), with a minimal DP of 2, see main text. To allow a further characterization of the OGAs, eluted fractions representing the different individual OGAs were isolated by a sodium acetate gradient, ranging from 0.01 M to 1 M, 0.1 M NaOH with a plateau of 10 min. at a concentration of 0.7 M on a semi-preparative CarboPac® PA-1 column.
Vorhölter et al. BMC Microbiology 2012 12:239 doi:10.1186/1471-2180-12-239