MALDI-TOF MS of oligosaccharides released from C. annuum cell walls by co-incubation with X. campestris pv. campestris. Cell walls of C. annuum and bacteria were co-incubated over night and the cell-free supernatant was desalted and lyophilized. This material was applied to MALDI-TOF MS using the negative-ion mode. A characteristic ladder of negatively charged ions was obtained. Mass differences correspond to that of OGAs of different degrees of polymerization (DP). Ions that correspond to DP 7 to 12 are indicated.
Vorhölter et al. BMC Microbiology 2012 12:239 doi:10.1186/1471-2180-12-239